ASPP2 Rabbit Polyclonal Antibody

SKU: orb10146

Description

ASPP2 Rabbit Polyclonal Antibody

Research Area

p53 Signaling Pathway

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ASPP2 (1001-1128/1128aa)
• Target: TP53BP2
• Molecular Weight: 124 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis-stimulating of p53 protein 2; Bbp; Bcl2-binding protein; NY-REN-51 antigen; p53-binding protein 2; p53BP2; PPP1R13A; TP53BP2; Tumor suppressor p53-binding protein 2; apoptosis stimulating protein of P53 2; ASPP2_HUMAN.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ASPP2) polyclonal Antibody, Unconjugated (orb10146) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Prostate (Mouse) Lysate at 30 ug, Small intestinal (Mouse) Lysate at 30 ug, Primary: Anti-ASPP2 (orb10146) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.

Quick Database Links

Gene Symbol

TP53BP2

UniProt

Q13625

Xie, Xiao-Feng et al. Prognostic values of apoptosis-stimulating P53-binding protein 1 and 2 and their relationships with clinical characteristics of esophageal squamous cell carcinoma patients: a retrospective study Chin J Cancer, 36, 15 (2017)

PubMed: 28103919