ASPP2 Rabbit Polyclonal Antibody

• Catalog Number: orb10146
• Category: Antibodies
• Description: ASPP2 Rabbit Polyclonal Antibody
• Target: TP53BP2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ASPP2 (1001-1128/1128aa)
• UniProt ID: Q13625
• RRID: AB_10747830
• MW: 124 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Apoptosis-stimulating of p53 protein 2; Bbp; Bcl2-
• Note: For research use only

References

Xie, Xiao-Feng et al. Prognostic values of apoptosis-stimulating P53-binding protein 1 and 2 and their relationships with clinical characteristics of esophageal squamous cell carcinoma patients: a retrospective study Chin J Cancer, 36, 15 (2017)

PubMed: 28103919

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ASPP2) polyclonal Antibody, Unconjugated (orb10146) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Prostate (Mouse) Lysate at 30 ug, Small intestinal (Mouse) Lysate at 30 ug, Primary: Anti-ASPP2 (orb10146) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000 dilution, Predicted band size: 124 kD, Observed band size: 124 kD.