ARSA Antibody

• Catalog Number: orb308772
• Category: Antibodies
• Description: ARSA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53588 MW
• UniProt ID: P15289
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Arylsulfatase A;ASA;3.1.6.8;Cerebroside-sulfatase;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-ARSA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-ARSA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ARSA using anti-ARSA antibody.Lane 1:Rat Testis Tissue;2:Rat Pancreas Tissue;3:Rat Skeletal Muscle Tissue;4:Mouse Kidney Tissue;5:MCF-7 Cell.

IF analysis of ARSA using anti-ARSA antibody. ARSA was detected in immunocytochemical section of U20S cell.

IHC analysis of ARSA using anti-ARSA antibody. ARSA was detected in paraffin-embedded section of Rat Lung Tissue.

IHC analysis of ARSA using anti-ARSA antibody. ARSA was detected in paraffin-embedded section of Human Mammary Cancer Tissue.