ARSA Antibody (monoclonal, 4C10)

• Catalog Number: orb443142
• Category: Antibodies
• Description: ARSA Antibody (monoclonal, 4C10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C10
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54 kDa
• UniProt ID: P15289
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Arylsulfatase A; ASA; Cerebroside-sulfatase; Aryls
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of ANA-1 cells using anti-ARSA antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of Raji cells using anti-ARSA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ARSA using anti-ARSA antibody.Lane 1:rat testis Tissue;2:rat liver Tissue;3:rat brain Tissue;4:rat lung Tissue;5:mouse testis Tissue;6:mouse liver Tissue;7:mouse brain Tissue;8:mouse lung Tissue;9:mouse HEPA1-6 Cell.

WB analysis of ARSA using anti-ARSA antibody.Lane 1:human A375 Cell;2:human A549 Cell;3:human SMMC-7721 Cell.

IHC analysis of ARSA using anti-ARSA antibody.ARSA was detected in paraffin-embedded section of human lung cancer tissue.