You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1522791 |
---|---|
Category | Antibodies |
Description | ARS2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 826 and 876 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ARS2 |
UniProt ID | Q9BXP5 |
NCBI | NP_056992.4 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | serrate; serrate RNA effector molecule homolog; AS Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse ARS2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARS2 antibody orb1522791 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human ARS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARS2 antibody orb1522791 used for IP at 6 µg per reaction.
Detection of human ARS2 by immunohistochemistry.Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti-ARS2 (Cat. No. orb1522791) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
Detection of mouse ARS2 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-ARS2 (Cat. No. orb1522791) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Porcine | |
Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
Filter by Rating