SRRT/ARS2 Antibody

• Catalog Number: orb1248063
• Category: Antibodies
• Description: SRRT/ARS2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-DPRAIVEYRDLDAPD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other commercial sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 140kDa
• Target: SRRT / ARS2
• UniProt ID: Q9BXP5
• NCBI: NP_001122325.1, NP_056992.4, NP_001122324.1, NP_001122326.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SRRT, serrate RNA effector molecule homolog (Arabi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other commercial sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248063 (0.1 ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248063 (0.1 ug/ml) staining of Rat Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248063 (5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.