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Aromatase Rabbit Polyclonal Antibody

Catalog Number: orb10506

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10506
CategoryAntibodies
DescriptionAromatase Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Aromatase (41-140/503aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW58 kDa
TargetCYP19A1
UniProt IDP11511
RRIDAB_10751792
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCytochrome p450 19A1; Estrogen synthetase; ARO 1;
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NoteFor research use only
Expiration Date12 months from date of receipt.
Aromatase Rabbit Polyclonal Antibody

Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-CYP19 antibody (orb10506), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10506, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Aromatase Rabbit Polyclonal Antibody

Sample: HepG2 Cell Lysate at 40 ug, Primary: Anti-CYP19 (orb10506) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 60 kD.

Aromatase Rabbit Polyclonal Antibody

Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYP19/CYP19A1 Polyclonal Antibody, Unconjugated (orb10506) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Aromatase Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYP19/CYP19A1 Polyclonal Antibody, Unconjugated (orb10506) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    ELISA,  IF,  IHC-P,  WB

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