CYP19A1 Antibody

SKU: orb1260791

Description

CYP19A1 Antibody

Research Area

Cancer Biology, Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2).
• Target: CYP19A1
• Molecular Weight: Observed: 48kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1, ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase

Western blot analysis of extracts of HeLa cells, using Aromatase antibody (orb1260791) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human colon using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Aromatase antibody (orb1260791) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CYP19A1

UniProt

P11511