ARHGEF4 antibody

• Catalog Number: orb18934
• Category: Antibodies
• Description: Goat polyclonal antibody to ARHGEF4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 79.1; 76.4
• Target: ARHGEF4
• Entrez: 50649
• Protein Sequence: PWEEPAGEKPSCS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ARHGEF4 antibody, anti Rho guanine nucleotide
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18934 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ARHGEF4 (RC215591) and probed with orb18934 overexpressing ARHGEF4 (RC215591) and probed with orb18934 (mock transfection in first lane), tested by Origene. (mock transfection in first lane), tested by Origene.