ARHGAP32 Rabbit Polyclonal Antibody

• Catalog Number: orb1859
• Category: Antibodies
• Description: ARHGAP32 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ARHGAP32 (801-920/2078aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 230 kDa
• Target: ARHGAP32
• UniProt ID: A7KAX9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Brain-specific Rho GTPase-activating protein; GAB-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sipei Chen 1, Li Wang 1, Yang Zou 1, Yi Li 1, Xiang Zhong 1, Guisen Li Pathogenesis of Focal Segmental Glomerulosclerosis Caused by a Leu754Val Mutation in ARHGAP32 FASEB J ., (2025)

PubMed: 40231370

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-ARHGAP32 antibody (orb1859), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.