ARFGAP1 Antibody

• Catalog Number: orb389390
• Category: Antibodies
• Description: ARFGAP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 44668 MW
• UniProt ID: Q8N6T3
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ADP-ribosylation factor GTPase-activating protein
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-ARFGAP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of ARFGAP1 using anti-ARFGAP1 antibody.Lane 1:human MOLT-4 cell;2:human Caco-2 cell;3:human HepG2 cell;4:rat brain tissue;5:mouse brain tissue.

IF analysis of ARFGAP1 using anti-ARFGAP1 antibody. ARFGAP1 was detected in an immunocytochemical section of CACO-2 cells.

IHC analysis of ARFGAP1 using anti-ARFGAP1 antibody. ARFGAP1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of ARFGAP1 using anti-ARFGAP1 antibody. ARFGAP1 was detected in a paraffin-embedded section of rat brain tissue.