ARF4 Antibody

SKU: orb1248657

Description

ARF4 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to Human NP_001651.1 and 20.4kDa according to Mouse NP_031505.1 ). Recommended concentration: 0.3-1µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SEMTDKLGLQSLRN
• Target: ARF4
• Molecular Weight: Approx 19 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARF4, ADP-ribosylation factor 4, ARF2, ADP-ribosylation factor 2, OTTHUMP00000213140, OTTHUMP00000213142, OTTHUMP00000213211

orb1248657 (0.5 ug/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248657 (5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ARF4

UniProt

P18085

RefSeq (Protein):NP_001651.1