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Catalog Number | orb2295919 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody raised against a full-length human ARAF protein. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, PLA, WB |
Reactivity | Human |
Immunogen | ARAF (NP_001645.1, 1 a.a. ~ 606 a.a) full-length human protein. |
Conjugation | Unconjugated |
Protein Sequence | MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP |
NCBI | NP_001645.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ARAF MaxPab rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.
Immunofluorescence of purified MaxPab antibody to ARAF on HeLa cell. [antibody concentration 30 ug/ml].
Proximity Ligation Analysis of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF MaxPab polyclonal antibody. Lane 1: ARAF transfected lysate(67.60 KDa). Lane 2: Non-transfected lysate.