Aquaporin 4 Antibody

• Catalog Number: orb1906388
• Category: Antibodies
• Description: Aquaporin 4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: D15107
• Tested applications: ELISA, FA, FC, IP
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Aquaporin 4
• UniProt ID: P55087
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-in
• Note: For research use only
• Application notes: This antibody binds extracellular domain of human aquaporin 4 with a very high binding affinity. The binding of this antibody inhibits the binding of NMO-IgG to AQP4 (PMID: 23746426). The binding affinity of this antibody for human AQP4 M23 isoform expressed on the surface of baculovirus and CHO cells was tested with ELISA (PMID: 26398585; WO2014196658). The binding characterization of this antibody was done using flow cytometry. This antibody can be used for the immunoprecipitation of human AQP4 but not mouse AQP4 (WO2014196658; PMID: 23909421). This antibody is not suitable for detection of denatured human AQP4 in a western blot assay (WO2014196658). Live imaging using Alexa‐Fluor‐555‐labelled antibodies revealed that this antibody more rapidly bound to cells expressing human aquaporin‐4 and strongly enhanced endocytosis and lysosomal degradation of AQP4, especially in cells expressing the M1 isoform alone. Chimeric D15107 prevented complement‐dependent cytotoxicity induced by NMO‐IgG from patient sera in vitro (PMID: 26398585).
• Expiration Date: 12 months from date of receipt.