AQP4 Antibody

• Catalog Number: orb1258025
• Category: Antibodies
• Description: AQP4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin 4 (NP_001641.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 36kDa
• Target: AQP4
• UniProt ID: P55087
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: MIWC, WCH4, aquaporin-4, aquaporin type4, mercuria
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Aquaporin 4 antibody (orb1258025) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat lung using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using Aquaporin 4 antibody (orb1258025) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of mouse brain using Aquaporin 4 Polyclonal Antibody (orb1258025) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.