Apom Antibody

SKU: orb1246450

Description

Apom Antibody

Research Area

Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu and Dahlbäck, J Biol Chem. 1999 Oct 29;274(44):31286-90.; PMID: 10531326). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KGNMELRTEGRPD
• Target: Apom
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apom, apolipoprotein M, G3a, NG20, 1190010O19Rik

orb1246450 (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246450 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Apom

UniProt

Q9Z1R3

RefSeq (Protein):NP_061286.1