Apolipoprotein D Rabbit Polyclonal Antibody

• Catalog Number: orb10114
• Category: Antibodies
• Description: Apolipoprotein D Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human APOD (81-189/189aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 19 kDa
• Target: APOD
• UniProt ID: P05090
• RRID: AB_10762611
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Apolipoprotein D; APOD; APO D; APOD protein; APOD_
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: A549 (Human) Cell Lysate at 30 ug Primary: Anti-Apolipoprotein D (orb10114) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 30 kD.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-APOD Polyclonal Antibody, Unconjugated (orb10114) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.