Apolipoprotein AI antibody

• Catalog Number: orb345275
• Category: Antibodies
• Description: Apolipoprotein AI antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:20,000, IHC: 1:50 - 1:200, IF: User Optimized, IP: 1:100, WB: 1:1,000 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Conjugation: Unconjugated
• UniProt ID: P02647
• NCBI: 4557321
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Apolipoprotein A-I Antibody, goat anti-A
• Note: For research use only
• Application notes: Anti-apoLipoprotein antibodies have been tested by Western blot and IHC and are suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human liver tissue using Apolipoprotein AI antibody

Western blot analysis of human serum prior to washing and elution of bound proteins using Apolipoprotein AI antibody