Apolipoprotein A I/APOA1 Antibody

• Catalog Number: orb371650
• Category: Antibodies
• Description: Apolipoprotein A I/APOA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human APOA1 recombinant protein (Position: D25-Q267). Human APOA1 shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat APOA1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA , 0.1-0.5μg/ml, Human, -Western blot, 0.1-0.5μg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30778 MW
• UniProt ID: P02647
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-APOA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of CACO-2 cells using anti-APOA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of APOA1 using anti-APOA1 antibody.Lane 1:human placenta tissue.

IHC analysis of APOA1 using anti-APOA1 antibody. APOA1 was detected in a paraffin-embedded section of human renal cancer tissue.

IHC analysis of APOA1 using anti-APOA1 antibody. APOA1 was detected in a paraffin-embedded section of human liver cancer tissue.