APOH Antibody / Apolipoprotein H

• Catalog Number: orb2636395
• Category: Antibodies
• Description: Human apolipoprotein H (apoH, also designated b2-glycoprotein I, activated protein C binding protein or APC inhibitor) is a five-domain plasma membrane-adhesion protein that is rich in sialic acid linked a to galactose or N-acetylgal-actosamine. apoH has been implicated in a variety of physiological pathways, including blood coagulation and the immune response. apoH is a cofactor for the binding of serum auto-antibodies from antiphospholipid syndrome, and is correlated with thrombosis, lupus erythematosus and recurrent fetal loss. In addition, apoH is also implicated in the clearance of apoptotic bodies from the circulation. The apoH gene is located on human chromosome 17q24.2. apoH is synthesized by hepatocytes and is present in blood associated with plasma lipoproteins. apoH displays a genetically determined structural polymorphism including three alleles (apoH*1, apoH*2 and apoH*3). apoH can inhibit the translocation of cholesterol from extracellular pools to macrophages, which reduces the cellular accumulation of cholesterol, suggesting that apoH may play an important role in the prevention of atherosclerosis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2c, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-300) of human APOH protein was used as the immunogen for the Apolipoprotein H antibody.
• UniProt ID: P02749
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Apolipoprotein H antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: APOH/3682
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Apolipoprotein H antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human liver tissue with Apolipoprotein H antibody (clone APOH/3682). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Apolipoprotein H antibody (clone APOH/3682) as confirmation of integrity and purity.