APOBEC3C Rabbit Polyclonal Antibody

• Catalog Number: orb155695
• Category: Antibodies
• Description: APOBEC3C Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human APOBEC3C (121-190/190aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: APOBEC3C
• UniProt ID: Q9NRW3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ABC3C_HUMAN; APOBEC1 like; APOBEC1-like; APOBEC1L;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4% Paraformaldehyde-fixed HepG2 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (APOBEC3C) polyclonal Antibody, unconjugated (orb155695) 1:50, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (APOBEC3C) Polyclonal Antibody, Unconjugated (orb155695) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.