APOBEC3B Rabbit Polyclonal Antibody

SKU: orb500784

Description

APOBEC3B Rabbit Polyclonal Antibody

Research Area

Anti-Viral Signaling, DNA / RNA Binding, DNA and RNA Research, Immunology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human APOBEC3B (1-100/382aa)
• Target: APOBEC3B
• Molecular Weight: 46 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APOBEC1L; Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B; apolipoprotein B mRNA editing enzyme, catalytic; ARCD3; ARP4; bK150C2.2; Cytidine deaminase; DJ742C19.2; FLJ21201; Phorbolin 1 related protein; Phorbolin 2; Phorbolin 2/3; Phorbolin 3; PHRBNL; Probable DNA dC>dU editing enzyme APOBEC3B.

Sample: jurkat (human) cell Lysate at 40 ug, Primary: Anti-APOBEC3B (orb500784) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46kD, Observed band size: 46 kD.

Tissue/Cell: Mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

APOBEC3B

UniProt

Q9UH17