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Catalog Number | orb1930104 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB4994 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 28996 Da |
Target | This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human APH1. |
UniProt ID | Q96BI3 |
NCBI | NP_057106.2, NP_001071096.1, NP_001230701.1, NP_001230700.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-APH1 Antibody (N-term) in A2058 cell line lysates (35 ug/lane). APH1 (arrow) was detected using the purified Pab.