APEX1 Antibody

• Catalog Number: orb1274460
• Category: Antibodies
• Description: APEX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 39kDa
• Target: APEX1
• UniProt ID: P27695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using APEX1 antibody (orb1274460). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (orb1274460) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using APEX1 antibody (orb1274460). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using APEX1 antibody (orb1274460).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug APEX1 antibody (orb1274460). Western blot was performed from the immunoprecipitate using APEX1 antibody (orb1274460) at a dilution of 1:1000.