APE1/APEX1 Antibody

• Catalog Number: orb215883
• Category: Antibodies
• Description: APE1/APEX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2 μg/ml, Human Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35555 MW
• UniProt ID: P27695
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA- (apurinic or apyrimidinic site) lyase;3.1.-.-
• Note: For research use only
• Application notes: WB: The detection limit for APE1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-APEX1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of APEX1 using anti-APEX1 antibody.Lane 1:Recombinant Human APEX1 Protein 0.5ng.

WB analysis of APEX1 using anti-APEX1 antibody.Lane 1:NRK Cell;2:HELA Cell;3:PC-12 Cell;4:RH35 Cell;5:HEPA Cell;6:MCF Cell;7:A549 Cell;8:Human Placenta Tissue;9:A431 Cell.

IF analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in immunocytochemical section of U20S cell.

IF analysis of APEX1 using anti-APEX1 antibody. APEX1 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of rat brain tissue.