Apelin Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb526574
• Category: Antibodies
• Description: Apelin Receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Canine, Equine, Gallus, Rabbit
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Apelin Receptor (181-280/380aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: APLNR
• UniProt ID: P35414
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: APJ receptor; AGTR L1; AGTRL 1; AGTRL1; Angiotensi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Apelin Receptor (orb526574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Apelin Receptor) Polyclonal Antibody, Unconjugated (orb526574) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Lung (Rat) Lysate at 40 ug, Lane 2: Spleen (Rat) Lysate at 40 ug, Primary: Anti-Apelin Receptor (orb526574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.