APEH Antibody

• Catalog Number: orb1257264
• Category: Antibodies
• Description: APEH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 81kDa
• Target: APEH
• UniProt ID: P13798
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: APEH, ACPH, APH, D3F15S2, D3S48E, DNF15S2, MGC2178
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using APEH antibody (orb1257264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug APEH antibody (orb1257264). Western blot was performed from the immunoprecipitate using APEH antibody (orb1257264) at a dilution of 1:1000.