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Catalog Number | orb1257264 |
---|---|
Category | Antibodies |
Description | APEH Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 81kDa |
Target | APEH |
UniProt ID | P13798 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | APEH, ACPH, APH, D3F15S2, D3S48E, DNF15S2, MGC2178 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using APEH antibody (orb1257264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 100 ug extracts of SW480 cells using 3 ug APEH antibody (orb1257264). Western blot was performed from the immunoprecipitate using APEH antibody (orb1257264) at a dilution of 1:1000.
IHC-P, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Equine, Porcine, Rabbit, Rat, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |