APE1 APEX1 Antibody (monoclonal, 5C11)

• Catalog Number: orb421116
• Category: Antibodies
• Description: APE1 APEX1 Antibody (monoclonal, 5C11)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5C11
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39 kDa
• UniProt ID: P27695
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA- (apurinic or apyrimidinic site) lyase; APEX n
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of APE1 using anti-APE1 antibody.Lane 1:human HeLa cell;2:human MCF-7 cell;3:human COLO-320 cell;4:human HepG2 cell;5:Rabbit IgG;6:Marker 1113;7:human A549 cell;8:human PANC-1 cell;9:human 22RV1 cell;10:human MDA-MB-453 cell.

IHC analysis of APE1 using anti-APE1 antibody.APE1 was detected in paraffin-embedded section of human intestinal cancer tissue.