ANAPC1, TSG24 Antibody

SKU: orb345494

Description

APC1 (phospho-S355) antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:10,000 - 1:35,000, IP: 1:100, WB: 1:200 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.
• Target: ANAPC1, TSG24
• Purity: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS355 residue. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from chimpanzee and chicken is expected as the sequence of the immunogen only varies by one amino acid in from these sources (89% homology). Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-APC1 pS355 Antibody, Anaphase-promoting complex subunit 1, APC-1, APC 1, Cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator

Quick Database Links

Gene Symbol

ANAPC1, TSG24

UniProt

Q9H1A4

RefSeq:12056971