APAF1 (NT) Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb15107
• Category: Antibodies
• Description: APAF1 (NT) Rabbit Polyclonal Antibody (FITC)
• Target: APAF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Apaf-1 (13-80/1248aa)
• RRID: AB_10756640
• Tested applications: FC, ICC, IF
• Dilution range: Flow-Cyt=0.2ug/test, IF=1:100-500, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: APAF 1; APAF1; APAF-1; Apoptotic peptidase activat
• Research Area: Apoptosis, Cell Biology, Cell Cycle, Cytochrome c,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-APAF1 (NT) antibody (orb15107), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-APAF1 (NT) antibody (orb15107), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.