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Item 1 of 2
APAF1 (NT) Rabbit Polyclonal Antibody (FITC)
Catalog Number: orb15107
| Catalog Number | orb15107 |
|---|---|
| Category | Antibodies |
| Description | APAF1 (NT) Rabbit Polyclonal Antibody (FITC) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, ICC, IF |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Apaf-1 (13-80/1248aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | Flow-Cyt=0.2ug/test, IF=1:100-500, ICC/IF=1:100 |
| Conjugation | FITC |
| Target | APAF1 |
| RRID | AB_10756640 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | APAF 1; APAF1; APAF-1; Apoptotic peptidase activat Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-APAF1 (NT) antibody (orb15107), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U937. Primary Antibody (green line): Rabbit Anti-APAF1 (NT) antibody (orb15107), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
