APAF1 Antibody

• Catalog Number: orb1240240
• Category: Antibodies
• Description: APAF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1.The sequences of the immunogenic peptide are identical between human and mouse.The immunogen is located within the first 50 amino acids of Apaf1.
• Concentration: 1 mg/ml
• Dilution range: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 132 kDa Observed: 130 kDa
• Target: APAF1
• UniProt ID: O14727
• NCBI: AAC51678
• Storage: Apaf1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic
• Note: For research use only
• Application notes: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 µg/mL.

Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 µg/mL.