ANXA3 monoclonal antibody (M12), clone 4F1

• Catalog Number: orb2296053
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant ANXA3.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: ANXA3 (AAH00871, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD
• Tested applications: ELISA, WB
• Clone Number: 4F1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH00871

Detection limit for recombinant GST tagged ANXA3 is approximately 1 ng/ml as a capture antibody.

Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1. Lane 1: ANXA3 transfected lysate(37.145 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of ANXA3 over-expressed 293 cell line, cotransfected with ANXA3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA3 monoclonal antibody (M12), clone 4F1. GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (61.27 KDa).