ANXA11 antibody

• Catalog Number: orb377923
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ANXA11.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Annexin A11
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ANXA11
• Entrez: 311, 11744
• UniProt ID: P50995, P97384
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ANX11; Annexin A11; 56 kDa autoantigen; Annexin XI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Annexin A11 expression in Jurkat (A), SKOV3 (B), A549 (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 51; 54 kD; Observed band size: 56 kD)

Immunofluorescent analysis of Annexin A11 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.