Annexin A11 antibody

• Catalog Number: orb18454
• Category: Antibodies
• Description: Goat polyclonal to Annexin A11
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 54.4
• Target: Annexin A11
• Entrez: 311
• Protein Sequence: YPGYPPPPGGYPP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ANXA11 antibody, anti annexin A11 antibody, a
• Note: For research use only
• Application notes: NP_001148.1; NP_665875.1 and NP_665876.1 are varients that encode the same protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong staining of Basement Membrane in trophoblasts. Recommended concentration, 3-5ug/ml.WB: Approx 55kDa band seen in HeLa lysate (Predicted MW of approx. 58kDa according to NP_001148.1). Recommended for use at 0.2-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18454 staining (0.2ug/ml) of HeLa lysate (RIPA buffer, 30ug total proteinper lane). Primary incubated for 1 hour. Detected by western blot usingchemiluminescence.

orb18454 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18454 (0.01µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.