ZWINT Rabbit Polyclonal Antibody

SKU: orb745935

Description

Anti-ZWINT Antibody. Tested in ELISA, WB applications. This antibody reacts with Rat.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Rat ELISA, 0.1-0.5μg/ml,
• Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat ZWINT recombinant protein (Position: M1-D253).
• Target: Outer kinetochore KNL1 complex subunit ZWINT
• Molecular Weight: 38 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zwint; Sip30; ZW10 interactor; SNAP25-interacting protein 30; Scoilin; ZW10-interacting protein 1; Zwint-1

Western blot analysis of ZWINT using anti-ZWINT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38 KD. The expected band size for ZWINT is at 38 KD.

Quick Database Links

Gene Symbol

Outer kinetochore KNL1 complex subunit ZWINT

UniProt

Q8VIL3