Vitamin D Binding protein/Gc Antibody

SKU: orb402288

Description

Anti-Vitamin D Binding protein/Gc Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Application Notes:
  Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse Gc recombinant protein (Position: L17-E256).
• Molecular Weight: 53 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc

Western blot analysis of Gc using anti-Gc antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse liver tissue lysate, Lane 4: mouse liver tissue lysate, Lane 5: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gc antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Gc at approximately 53KD. The expected band size for Gc is at 53KD.

Quick Database Links

UniProt

P21614