You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb402236 |
---|---|
Category | Antibodies |
Description | Anti-VCAM1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human VCAM1 recombinant protein (Position: F25-L270). |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 95 kDa |
UniProt ID | P19320 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; Read more... |
Note | For research use only |
Application notes | Western blot, 0.1-0.5μg/ml ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Sandwich ELISA - Recombinant human VCAM1 protein standard curve. Use in combination with reagents from Human VCAM1 ELISA Kit EZ-Set (DIY Antibody Pairs).
Western blot analysis of VCAM1 using anti-VCAM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human VCAM1 protein 1 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 95KD. The expected band size for VCAM1 is at 74KD.