Anti-V5-tag Antibody

• Catalog Number: orb1421529
• Category: Antibodies
• Description: Mouse monoclonal antibody to V5-tag.
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Dilution range: WB: WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200), IF: WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200), IP: WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of over-expressed V5-tagged protein in 293T cell lysate.

Immunoprecipitation of V5-tagged protein from HEK293T cells transfected with vector overexpressing V5 tag, using Anti-V5-tag Antibody.

Immunofluorescent analysis of V5-tag staining in 293T cells transfected with a V5-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).