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Anti-UBCH7 Antibody

Catalog Number: orb382106

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb382106
CategoryAntibodies
DescriptionRabbit polyclonal antibody to UBE2L3.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human UBCH7
Antibody TypePrimary Antibody
Dilution rangeWB: 1:1000-2000, IHC-P: 1:50-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetUBE2L3
Entrez7332, 22195, 100042355
UniProt IDP68037, P68036
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesUBCE7; UBCH7; Ubiquitin-conjugating enzyme E2 L3;
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-UBCH7 Antibody

Western blot analysis of UBCH7 expression in A549 (A), K562 (B), mouse testis (C), mouse brain (D) whole cell lysates. (Predicted band size: 14; 17; 24 kD; Observed band size: 18 kD)

Anti-UBCH7 Antibody

Immunohistochemical analysis of UBCH7 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-UBCH7 Antibody

Immunofluorescent analysis of UBCH7 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    ELISA,  IF,  WB

    Bovine, Human, Mouse, Porcine, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
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    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    IHC,  WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    IHC,  IP,  WB

    Human, Mouse

    Rabbit

    Polyclonal

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