UBA3 Antibody

• Catalog Number: orb668550
• Category: Antibodies
• Description: The UBA3 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human UBA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human UBA3. The exact sequence is proprietary.
• UniProt ID: Q99MI7, Q8TBC4, Q8C878
• Tested applications: IHC, WB
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: UBE1C; NEDD8-activating enzyme E1 catalytic subuni
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 117553, 9039, 22200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of UBA3 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 53 kD)

Immunohistochemical analysis of UBA3 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.