Anti-U2AF65 Antibody

• Catalog Number: orb340799
• Category: Antibodies
• Description: Rabbit polyclonal antibody to U2AF2
• Target: U2AF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human U2AF65
• UniProt ID: P26369, P26368
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: U2AF65; Splicing factor U2AF 65 kDa subunit; U2 au
• Note: For research use only
• Entrez: 22185, 11338

Western blot analysis of U2AF65 expression in Jurkat (A), SW620 (B), mouse spleen (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)

Immunofluorescent analysis of U2AF65 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.