Anti-TRPC5 Antibody

• Catalog Number: orb234317
• Category: Antibodies
• Description: Anti-TRPC5 Antibody. Tested in WB applications. This antibody reacts with Human.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human TRPC5 recombinant protein (Position: K684-L973). Human TRPC5 shares 90% amino acid (aa) sequence identity with mouse TRPC5.
• UniProt ID: Q9UL62
• MW: 111 kDa
• Tested applications: WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Short transient receptor potential channel 5; TrpC
• Note: For research use only

Anti-TRPC5 Picoband antibody, Western blotting. All lanes: Anti TRPC5 at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: U87 Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Predicted bind size: 111 KD. Observed bind size: 111 KD.

Anti-TRPC5 Picoband antibody, Western blotting. All lanes: Anti TRPC5 at 0.5 ug/ml. WB: Recombinant Human TRPC5 Protein 0.5 ng. Predicted bind size: 50 KD. Observed bind size: 50 KD.

Western blot analysis of TRPC5 using anti-TRPC5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antibody at 1:200 overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using a fluorescent. A specific band was detected for TRPC5 at approximately 111 kDa. The expected band size for TRPC5 is at 111 kDa.