TRAF2 Rabbit Polyclonal Antibody

SKU: orb308811

Description

TRAF2 Rabbit Polyclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Western blot, 0.1-0.5μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF2, different from the related mouse sequence by one amino acid.
• Target: TNF receptor-associated factor 2
• Molecular Weight: 53 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNF receptor-associated factor 2; 6.3.2.-; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3; TRAF2; TRAP3

IHC analysis of TRAF2 using anti-TRAF2 antibody. TRAF2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-TRAF2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of TRAF2 using anti-TRAF2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50 ug, Lane 2: Rat Testis Tissue Lysate at 50 ug, Lane 3: Mouse Brain Tissue Lysate at 50 ug, Lane 4: Human Placenta Tissue Lysate at 50 ug, Lane 5: 22RV1 Whole Cell Lysate at 40 ug, Lane 6: SMMC Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 53 kDa. The expected band size for TRAF2 is at 53 kDa.

Quick Database Links

Gene Symbol

TNF receptor-associated factor 2

UniProt

Q12933