Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab)

• Catalog Number: orb1818459
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human, Monkey
• Isotype: IgG4PE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 150 Da
• UniProt ID: P25942
• Protein Sequence: TNFRSF5 / CD40
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tumor necrosis factor receptor superfamily member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab) is more than 99.21%, determined by SEC-HPLC.

Immobilized human TNFRSF5 / CD40 His at 2 µg/mL can bind Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab), EC50=0.0252 µg/mL.

Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab).FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 1.359 ug/mL.

Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Human CD40 CHO cells were stained with Anti-TNFRSF5 / CD40 Reference Antibody (bleselumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC77=0.6311 ug/mL