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Anti-TIMP3 Antibody
Catalog Number: orb334563
| Catalog Number | orb334563 |
|---|---|
| Category | Antibodies |
| Description | Anti-TIMP3 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E. coli-derived human TIMP3 recombinant protein (Position: C24-P211). Human TIMP3 shares 97.9% and 97.3% amino acid (aa) sequence identity with mouse and rat TIMP3, respectively. |
| UniProt ID | P35625 |
| MW | 34 kDa |
| Tested applications | ELISA, WB |
| Application notes | ELISA, 0.1-0.5μg/ml, -Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Metalloproteinase inhibitor 3; Protein MIG-5; Tiss Read more... |
| Note | For research use only |
Western blot analysis of TIMP3 using anti-TIMP3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50 ug, Lane 2: Mouse Ovary Tissue Lysate at 50 ug, Lane 3: HELA Whole Cell Lysate at 40 ug, Lane 4: MCF-7 Whole Cell Lysate at 40 ug, Lane 5: SMMC Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 34 kDa. The expected band size for TIMP3 is at 34 kDa.
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