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Anti-TBR2/Eomes Antibody
Catalog Number: orb413021
| Catalog Number | orb413021 |
|---|---|
| Category | Antibodies |
| Description | Anti-TBR2/Eomes Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E. coli-derived human TBR2/Eomes recombinant protein (Position: T310-D532). |
| UniProt ID | O95936 |
| MW | 85 kDa |
| Tested applications | ELISA, WB |
| Application notes | Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Eomesodermin homolog; T-box brain protein 2; T-bra Read more... |
| Note | For research use only |
Western blot analysis of TBR2/Eomes using anti-TBR2/Eomes antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR2/Eomes antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TBR2/Eomes at approximately 85KD. The expected band size for TBR2/Eomes is at 73KD.
