Cart summary

You have no items in your shopping cart.

Anti-TAS1R2 Antibody

Catalog Number: orb1972563

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1972563
CategoryAntibodies
DescriptionAnti-TAS1R2 Antibody. Tested in WB, ELISA applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
ImmunogenE.coli-derived human TAS1R2 recombinant protein (Position: Y96-D839). Human TAS1R2 shares 69.6% and 71% amino acid (aa) sequence identity with mouse and rat TAS1R2, respectively.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW100 kDa
UniProt IDQ8TE23
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-TAS1R2 Antibody

Western blot analysis of TAS1R2 using anti-TAS1R2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human COLO 320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TAS1R2 at approximately 100 kDa. The expected band size for TAS1R2 is at 95 kDa.

  • Goat anti-TAS1R2 / GPR71 Antibody [orb19715]

    ELISA,  WB

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-TAS1R2 Antibody [orb2582292]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-TAS1R2 Antibody [orb2582293]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-TAS1R2 Antibody [orb2582294]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-TAS1R2 Antibody [orb2582295]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    HRP

    100 μg