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Catalog Number | orb1422690 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Tachykinin Receptor 1. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | TACR1 |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
Conjugation | Unconjugated |
UniProt ID | P25103 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | NK1R; TAC1R; Substance-P receptor; SPR; NK-1 recep Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of Tachykinin Receptor 1 staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Tachykinin Receptor 1 expression in HEK293T (A), A2780 (B), mouse spleen (C), mouse kidney (D), rat spleen (E), rat kidney (F) whole cell lysates.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |