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Anti-Sp7/Osterix Antibody
Catalog Number: orb738396
| Catalog Number | orb738396 |
|---|---|
| Category | Antibodies |
| Description | Anti-Sp7/Osterix Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Sp7/Osterix recombinant protein (Position: A2-E241). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 45 kDa |
| UniProt ID | Q8TDD2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Deleted in azoospermia-like; DAZ homolog; DAZ-like Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MDA-MB453 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. Lane 7: rat lung tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45 KD. The expected band size for Sp7/Osterix is at 45 KD.
