Anti-SIX4 Antibody

• Catalog Number: orb1743844
• Category: Antibodies
• Description: Anti-SIX4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human
• Immunogen: E.coli-derived human SIX4 recombinant protein (Position: Q387-L781).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 95 kDa
• UniProt ID: Q9UIU6
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-SIX4 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of SIX4 using anti-SIX4 antibody.Lane 1:human A431 cell;2:human RT4 cell;3:human U20S cell.

IF analysis of SIX4 using anti-SIX4 antibody and anti-Beta Tubulin antibody.SIX4 was detected in immunocytochemical section of U2OS cell.