Semaphorin 3E Rabbit Polyclonal Antibody

SKU: orb214925

Description

The Semaphorin 3E Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Semaphorin 3E. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Semaphorin 3E. The exact sequence is proprietary.
• Target: SEMA3E
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIAA0331; SEMAH; Semaphorin-3E

Western blot analysis of Semaphorin 3E expression in A549 (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 89 kD)

Immunofluorescent analysis of Semaphorin 3E staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

SEMA3E

UniProt

P70275, O15041

NCBI Gene ID

20349, 9723